Hotspot

trnK-UUU-rps16

LSC · 4335-4904 · 570 bp

Back to Sabulina

Pi 0.128

MarkerSeek score 81.80

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 570
variable_sites: 71
pi: 0.128
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCTTTCAGGATCAGTCGTGG	CACGATAAAGGCTTCCGGAA	55.0	50.0	58.0	58.0	1328-1386 bp	1.0	86.2
trnK-UUU-rps16_p2	CCTTTCAGGATCAGTCGTGG	CGATAAAGGCTTCCGGAACA	55.0	50.0	58.0	58.0	1326-1384 bp	1.0	86.2
trnK-UUU-rps16_p3	CCTTTCAGGATCAGTCGTGG	ACGATAAAGGCTTCCGGAAC	55.0	50.0	58.0	58.0	1327-1385 bp	1.0	86.1
trnK-UUU-rps16_p4	CGAATCCCTTGCTTCATCCA	CACGATAAAGGCTTCCGGAA	50.0	50.0	58.0	58.0	1281-1339 bp	1.0	86.1
trnK-UUU-rps16_p5	CGAATCCCTTGCTTCATCCA	CGATAAAGGCTTCCGGAACA	50.0	50.0	58.0	58.0	1279-1337 bp	1.0	86.1