Hotspot

rps16

LSC · 5184-6281 · 1098 bp

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Pi 0.0114
MarkerSeek score 53.40
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1098
variable_sites
29
pi
0.0114
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 TCAATCCCCTTTCTACCCGT AGCAATCCGTTGAAGATGCA 50.0 45.0 58.0 58.2 1520-1539 bp 1.0 80.9
rps16_p2 TCAATCCCCTTTCTACCCGT GGCTTTTACGGGTGATTGGA 50.0 50.0 58.0 58.2 1663-1682 bp 1.0 80.8
rps16_p3 TCAATCCCCTTTCTACCCGT GCAATCCGTTGAAGATGCAG 50.0 50.0 58.0 57.8 1519-1538 bp 1.0 80.8
rps16_p4 ACCCGTCTCACGTCTCATAT GGCTTTTACGGGTGATTGGA 50.0 50.0 57.9 58.2 1649-1668 bp 1.0 80.7
rps16_p5 TCAATCCCCTTTCTACCCGT TTCGGCTTTTACGGGTGATT 50.0 45.0 58.0 57.8 1666-1685 bp 1.0 80.7