Hotspot

ndhD

SSC · 122510-124012 · 1503 bp

Pi 0.00266

MarkerSeek score 41.50

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 14
pi: 0.00266
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GTGAATTCTGCAATCGTGGC	TAGTGCGTACAACAGACACG	50.0	50.0	58.1	58.0	2433-2506 bp	0.888889	77.7
ndhD_p2	CGCCAAACCTATCTGAGTCC	TATGGCATGAGACAACTCGC	55.0	50.0	58.1	58.1	1785-1837 bp	0.444444	59.8
ndhD_p3	CGCCAAACCTATCTGAGTCC	TAGTGCGTACAACAGACACG	55.0	50.0	58.1	58.0	2204-2256 bp	0.444444	59.6
ndhD_p4	CGCCAAACCTATCTGAGTCC	GAAATCAAAGCACCTTGGCC	55.0	50.0	58.1	57.9	2159-2211 bp	0.444444	59.3
ndhD_p5	CGCCAAACCTATCTGAGTCC	TCTCATGAGCAGACCCAGAA	55.0	50.0	58.1	58.1	2187-2190 bp	0.333333	52.8