Hotspot

rpl32-trnL-UAG

SSC · 120022-121048 · 1027 bp

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Pi 0.0119
MarkerSeek score 61.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1027
variable_sites
38
pi
0.0119
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 ACCGGGAATTCAAAAAGTTT AGCGTGTCTACCGATTTCAC 35.0 50.0 53.9 58.0 1094-1161 bp 1.0 52.1
rpl32-trnL-UAG_p2 ACCGGGAATTCAAAAAGTTT CAGCGTGTCTACCGATTTCA 35.0 50.0 53.9 58.0 1095-1162 bp 1.0 52.0
rpl32-trnL-UAG_p3 ACCGGGAATTCAAAAAGTTT TCCTAAGAGCAGCGTGTCTA 35.0 50.0 53.9 57.9 1104-1171 bp 1.0 51.1
rpl32-trnL-UAG_p4 ACCGGGAATTCAAAAAGTTTT AGCGTGTCTACCGATTTCAC 33.3 50.0 54.7 58.0 1094-1161 bp 1.0 51.1
rpl32-trnL-UAG_p5 ACCGGGAATTCAAAAAGTTTT CAGCGTGTCTACCGATTTCA 33.3 50.0 54.7 58.0 1095-1162 bp 1.0 51.1