Hotspot

psbZ-trnG-GCC

LSC · 39483-39825 · 343 bp

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Pi 0.00877
MarkerSeek score 59.80
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
343
variable_sites
10
pi
0.00877
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 TTGCTTCTCCTGATGGTTGG CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.8 57.8 500-529 bp 1.0 79.3
psbZ-trnG-GCC_p2 TTGCTTCTCCTGATGGTTGG CTTCACCATAGGCATAGGCG 50.0 55.0 57.8 58.2 540-569 bp 1.0 78.9
psbZ-trnG-GCC_p3 GCTTCTCCTGATGGTTGGTC CGTCTTCTCCTTGGCAAAGA 55.0 50.0 58.3 57.8 498-527 bp 1.0 78.9
psbZ-trnG-GCC_p4 TTGCTTCTCCTGATGGTTGG ACCATAGGCATAGGCGGATA 50.0 50.0 57.8 57.8 536-565 bp 1.0 78.6
psbZ-trnG-GCC_p5 GCTTCTCCTGATGGTTGGTC CTTCACCATAGGCATAGGCG 55.0 55.0 58.3 58.2 538-567 bp 1.0 78.5