Hotspot

ycf1

SSC · 127361-132979 · 5619 bp

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Pi 0.00117
MarkerSeek score 38.60
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5619
variable_sites
26
pi
0.00117
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
38.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TCTTGTCCCTGTTGTTCGTT TGGATGGGAATGAATCAAGA 45.0 40.0 57.3 54.0 284 bp 1.0 43.0
ycf1_p2 TTCTTGTCCCTGTTGTTCGT TGGATGGGAATGAATCAAGA 45.0 40.0 57.3 54.0 285 bp 1.0 43.0
ycf1_p3 TCTTGTCCCTGTTGTTCGTT TGATTGGATGGGAATGAATCA 45.0 38.1 57.3 54.9 288 bp 1.0 42.6
ycf1_p4 TTCTTGTCCCTGTTGTTCGT TGATTGGATGGGAATGAATCA 45.0 38.1 57.3 54.9 289 bp 1.0 42.6
ycf1_p5 TCTTGTCCCTGTTGTTCGTT GGATGGGAATGAATCAAGAAAAGG 45.0 41.7 57.3 58.1 283 bp 1.0 42.5