Hotspot

ndhF

SSC · 113888-116125 · 2238 bp

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Pi 0.000715
MarkerSeek score 46.70
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2238
variable_sites
8
pi
0.000715
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 ACGAAAAATGTCTTTGTACACGA TCGAAAGAGATAAGAATTGGTGA 34.8 34.8 57.3 55.4 2343 bp 1.0 40.3
ndhF_p2 ACGAAAAATGTCTTTGTACACGA AGAATTGGTGAATCAGAAACA 34.8 33.3 57.3 53.3 2331 bp 1.0 40.3
ndhF_p3 ACGAAAAATGTCTTTGTACACGA AAGAATTGGTGAATCAGAAACA 34.8 31.8 57.3 54.2 2332 bp 1.0 40.3
ndhF_p4 ACGAAAAATGTCTTTGTACACGA AGAATTGGTGAATCAGAAACAA 34.8 31.8 57.3 54.2 2331 bp 1.0 40.3
ndhF_p5 CGAAAAATGTCTTTGTACACGAC TCGAAAGAGATAAGAATTGGTGA 39.1 34.8 57.0 55.4 2342 bp 1.0 40.3