Hotspot

rpl32-trnL-UAG

SSC · 116596-117393 · 798 bp

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Pi 0.00201
MarkerSeek score 46.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
798
variable_sites
8
pi
0.00201
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGTTTCTACCGGACATTCAA AGCGTGTCTACCGATTTCAC 45.0 50.0 55.4 58.0 889-896 bp 1.0 59.9
rpl32-trnL-UAG_p2 GGTTTCTACCGGACATTCAA CAGCGTGTCTACCGATTTCA 45.0 50.0 55.4 58.0 890-897 bp 1.0 59.9
rpl32-trnL-UAG_p3 GGTTTCTACCGGACATTCAA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 55.4 57.9 899-906 bp 1.0 59.0
rpl32-trnL-UAG_p4 GGTTTCTACCGGACATTCAA CCTAAGAGCAGCGTGTCTAC 45.0 55.0 55.4 57.8 898-905 bp 1.0 58.4
rpl32-trnL-UAG_p5 GGTTTCTACCGGACATTCAA CTAAGAGCAGCGTGTCTACC 45.0 55.0 55.4 57.8 897-904 bp 1.0 58.4