Hotspot

rpl20-rps12

LSC · 71512-72223 · 712 bp

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Pi 0.000781
MarkerSeek score 46.40
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
712
variable_sites
2
pi
0.000781
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 CGACGTTTCCGAGCTGTATA TCTCCCGCTCTTAGAGGATG 50.0 55.0 57.5 58.0 806-807 bp 1.0 76.6
rpl20-rps12_p2 TCCGAGCTGTATATCCTCGT TCTCCCGCTCTTAGAGGATG 50.0 55.0 57.4 58.0 799-800 bp 1.0 75.8
rpl20-rps12_p3 CGACGTTTCCGAGCTGTATA GAGGAACATGCACTAGGGTG 50.0 55.0 57.5 58.0 775-776 bp 0.9 72.4
rpl20-rps12_p4 CGACGTTTCCGAGCTGTATA GTCGAGGAACATGCACTAGG 50.0 55.0 57.5 58.1 778-779 bp 0.9 72.0
rpl20-rps12_p5 TCCGAGCTGTATATCCTCGT GAGGAACATGCACTAGGGTG 50.0 55.0 57.4 58.0 768-769 bp 0.9 71.6