Hotspot

petA-psbJ

LSC · 65384-66391 · 1008 bp

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Pi 0.00335
MarkerSeek score 42.60
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1008
variable_sites
9
pi
0.00335
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
42.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 ATCCATTACGTGTCCAAGGC GGTTCATATTCCGGGTTGGG 50.0 55.0 58.0 58.3 1160-1174 bp 1.0 77.8
petA-psbJ_p2 ATCCATTACGTGTCCAAGGC CATATTCCGGGTTGGGTTCA 50.0 50.0 58.0 57.6 1156-1170 bp 1.0 76.9
petA-psbJ_p3 GGCCTTTTGTTCTTCTTGGC GGTTCATATTCCGGGTTGGG 50.0 55.0 57.8 58.3 1143-1157 bp 1.0 76.9
petA-psbJ_p4 ATCCATTACGTGTCCAAGGC GTTCATATTCCGGGTTGGGT 50.0 50.0 58.0 57.6 1159-1173 bp 1.0 76.9
petA-psbJ_p5 CATTACGTGTCCAAGGCCTT GGTTCATATTCCGGGTTGGG 50.0 55.0 58.2 58.3 1157-1171 bp 1.0 76.7