Hotspot

rbcL-accD

LSC · 59125-59907 · 783 bp

Back to Sabal

Pi 0.00312
MarkerSeek score 44.20
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
783
variable_sites
9
pi
0.00312
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 GCAAATGGAGCCCTGAACTA CAACATCGAATTGAACCACCA 50.0 42.9 57.9 57.1 899-903 bp 1.0 64.8
rbcL-accD_p2 TAGCAAATGGAGCCCTGAAC CAACATCGAATTGAACCACCA 50.0 42.9 57.9 57.1 901-905 bp 1.0 64.8
rbcL-accD_p3 GCAAATGGAGCCCTGAACTA ACAACATCGAATTGAACCACC 50.0 42.9 57.9 57.1 900-904 bp 1.0 64.8
rbcL-accD_p4 TAGCAAATGGAGCCCTGAAC ACAACATCGAATTGAACCACC 50.0 42.9 57.9 57.1 902-906 bp 1.0 64.8
rbcL-accD_p5 TGAAGCTAGCAAATGGAGCC CAACATCGAATTGAACCACCA 50.0 42.9 58.2 57.1 907-911 bp 1.0 63.9