Hotspot

trnC-GCA-petN

LSC · 28907-30013 · 1107 bp

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Pi 0.00137
MarkerSeek score 49.20
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
1107
variable_sites
6
pi
0.00137
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 GGGGGACTGCAAATCCTTTA TAAAGCAGCCCAAGTGAGAC 50.0 50.0 57.5 57.8 1183-1187 bp 1.0 75.0
trnC-GCA-petN_p2 GGGGGACTGCAAATCCTTTA ACCATTAAAGCAGCCCAAGT 50.0 45.0 57.5 57.7 1188-1192 bp 1.0 74.0
trnC-GCA-petN_p3 GGGGGACTGCAAATCCTTTA AGAGTCCACTTCTTCCCCAT 50.0 50.0 57.5 57.6 1238-1242 bp 1.0 73.6
trnC-GCA-petN_p4 CCAGTTCAAATCCGGGTGTC TAAAGCAGCCCAAGTGAGAC 55.0 50.0 58.8 57.8 1160-1164 bp 1.0 72.5
trnC-GCA-petN_p5 GGGGGACTGCAAATCCTTTA GCAGCCCAAGTGAGACTTAC 50.0 55.0 57.5 58.6 1179-1183 bp 1.0 72.0