Hotspot

trnS-GCU-trnG-UCC

LSC · 8418-9669 · 1252 bp

Back to Sabal

Pi 0.00244
MarkerSeek score 57.20
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
1252
variable_sites
14
pi
0.00244
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 ATTAGCAATCCGCCGCTTTA ACGAATCACACTTTTACCACT 45.0 38.1 58.0 55.5 1167-1350 bp 1.0 56.3
trnS-GCU-trnG-UCC_p2 ATTAGCAATCCGCCGCTTTA AGAACGAATCACACTTTTACCAC 45.0 39.1 58.0 57.3 1170-1353 bp 1.0 55.2
trnS-GCU-trnG-UCC_p3 ATTAGCAATCCGCCGCTTTA GAACGAATCACACTTTTACCACT 45.0 39.1 58.0 57.3 1169-1352 bp 1.0 55.2
trnS-GCU-trnG-UCC_p4 AGAAGAAGCGGAGAGAGAGG ACGAATCACACTTTTACCACT 55.0 38.1 58.2 55.5 1223-1406 bp 1.0 54.6
trnS-GCU-trnG-UCC_p5 ATTAGCAATCCGCCGCTTTA AACGAATCACACTTTTACCACT 45.0 36.4 58.0 56.2 1168-1351 bp 1.0 54.3