Hotspot

rps16-trnQ-UUG

LSC · 5972-7085 · 1114 bp

Back to Sabal

Pi 0.00122
MarkerSeek score 46.80
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1114
variable_sites
6
pi
0.00122
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CAGCCGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1205-1206 bp 1.0 79.6
rps16-trnQ-UUG_p2 GCCGATCATGTCCTTCAAGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1203-1204 bp 1.0 79.6
rps16-trnQ-UUG_p3 AATCCACAGCCGATCATGTC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1211-1212 bp 1.0 79.5
rps16-trnQ-UUG_p4 GAATCCACAGCCGATCATGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1212-1213 bp 1.0 79.5
rps16-trnQ-UUG_p5 CAGCCGATCATGTCCTTCAA GAAATTCGAAATGGGGCGTG 50.0 50.0 58.0 58.1 1264-1265 bp 1.0 79.4