Hotspot

rpoC1

LSC · 22238-24289 · 2052 bp

Back to Rytidosperma

Pi 0.00244

MarkerSeek score 44.80

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2052
variable_sites: 5
pi: 0.00244
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	CAACCCGTTAGAGGAAGAGC	GAAAACTCTGTTGTTCCGCG	55.0	50.0	58.0	58.0	2823 bp	1.0	80.0
rpoC1_p2	GGAGATCCCTTCGAACAACC	GAAAACTCTGTTGTTCCGCG	55.0	50.0	58.0	58.0	2940 bp	1.0	79.9
rpoC1_p3	CAACCCGTTAGAGGAAGAGC	TCGCAGTTCCGTCTATCTCT	55.0	50.0	58.0	58.0	2637 bp	1.0	79.9
rpoC1_p4	GGAGATCCCTTCGAACAACC	TCGCAGTTCCGTCTATCTCT	55.0	50.0	58.0	58.0	2754 bp	1.0	79.8
rpoC1_p5	ACAGGAGATCCCTTCGAACA	GAAAACTCTGTTGTTCCGCG	50.0	50.0	58.1	58.0	2943 bp	1.0	79.6