Hotspot

psbC

LSC · 9762-11183 · 1422 bp

Back to Rytidosperma

Pi 0.00211

MarkerSeek score 56.10

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 3
pi: 0.00211
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	TGTTTCCCAGGAAATCCGTG	TGGCTCGGCTAGATGGAATA	50.0	50.0	58.1	58.0	1604 bp	1.0	79.3
psbC_p2	TGTTTCCCAGGAAATCCGTG	AAAGAATGAATGGCTCGGCT	50.0	45.0	58.1	57.9	1614 bp	1.0	78.3
psbC_p3	GCAGCGGAAGATCCTGAATT	TGGCTCGGCTAGATGGAATA	50.0	50.0	58.3	58.0	1585 bp	1.0	77.5
psbC_p4	TGTTTCCCAGGAAATCCGTG	AACCGAGCCAGAACAGAAAA	50.0	45.0	58.1	57.7	1585 bp	1.0	76.7
psbC_p5	TGTTTCCCAGGAAATCCGTG	ACCGAGCCAGAACAGAAAAA	50.0	45.0	58.1	57.7	1584 bp	1.0	76.7