Hotspot

rpl32-trnL-UAG

SSC · 102980-103302 · 323 bp

Back to Rytidosperma

Pi 0.0217
MarkerSeek score 51.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
323
variable_sites
7
pi
0.0217
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGCAACGAGAATCCAAAACC GAAGAATCACGCAATCCCCT 50.0 50.0 57.7 58.0 668 bp 1.0 77.8
rpl32-trnL-UAG_p2 GGCAACGAGAATCCAAAACC GATCCGCACAAAAGAGTTGC 50.0 50.0 57.7 58.0 1007 bp 1.0 77.6
rpl32-trnL-UAG_p3 TCCCCTTTTTGAATGGCAGT GAAGAATCACGCAATCCCCT 45.0 50.0 57.6 58.0 806 bp 1.0 77.3
rpl32-trnL-UAG_p4 GGCAACGAGAATCCAAAACC ATGTCCCGAAGAAACCCAAC 50.0 50.0 57.7 58.1 741 bp 1.0 77.3
rpl32-trnL-UAG_p5 TCCCCTTTTTGAATGGCAGT GATCCGCACAAAAGAGTTGC 45.0 50.0 57.6 58.0 1145 bp 1.0 77.1