Hotspot

rpl14-rpl16

LSC · 76061-76163 · 103 bp

Back to Rytidosperma

Pi 0.0194

MarkerSeek score 63.70

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 103
variable_sites: 2
pi: 0.0194
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	GATTACCGGCAGCTCCTATG	GCCAGCAAAATGCCTATACG	55.0	50.0	57.9	57.6	242 bp	1.0	76.7
rpl14-rpl16_p2	GCTCCACTGTTATCCGCTAC	GCCAGCAAAATGCCTATACG	55.0	50.0	58.1	57.6	198 bp	1.0	76.4
rpl14-rpl16_p3	AGCTCCACTGTTATCCGCTA	GCCAGCAAAATGCCTATACG	50.0	50.0	58.2	57.6	199 bp	1.0	75.8
rpl14-rpl16_p4	ATTACCGGCAGCTCCTATGA	GCCAGCAAAATGCCTATACG	50.0	50.0	58.3	57.6	241 bp	1.0	75.2
rpl14-rpl16_p5	TGATTACCGGCAGCTCCTAT	GCCAGCAAAATGCCTATACG	50.0	50.0	58.3	57.6	243 bp	1.0	75.1