Hotspot

trnW-CCA-trnP-UGG

LSC · 62477-62607 · 131 bp

Back to Rytidosperma

Pi 0.0153

MarkerSeek score 61.30

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 131
variable_sites: 2
pi: 0.0153
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	ACCTACGACATCGGGTTTTG	CTTGGTAGCGCGTTTGTTTT	50.0	45.0	57.9	57.9	245 bp	1.0	79.0
trnW-CCA-trnP-UGG_p2	ACCTACGACATCGGGTTTTG	TTGGTAGCGCGTTTGTTTTG	50.0	45.0	57.9	58.2	244 bp	1.0	78.6
trnW-CCA-trnP-UGG_p3	TGGAATCACGCTCTGTAGGA	CTTGGTAGCGCGTTTGTTTT	50.0	45.0	58.2	57.9	270 bp	1.0	78.5
trnW-CCA-trnP-UGG_p4	ACCTACGACATCGGGTTTTG	CGCGTTTGTTTTGGGTACAA	50.0	45.0	57.9	57.8	237 bp	1.0	78.4
trnW-CCA-trnP-UGG_p5	TGGAATCACGCTCTGTAGGA	TTGGTAGCGCGTTTGTTTTG	50.0	45.0	58.2	58.2	269 bp	1.0	78.0