Hotspot

petA-psbJ

LSC · 59202-60141 · 940 bp

Back to Rytidosperma

Pi 0.00876

MarkerSeek score 66.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 940
variable_sites: 8
pi: 0.00876
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTCGC	TCTGGATTGGGTTCGTCTCT	50.0	50.0	57.9	58.1	1039-1066 bp	1.0	81.8
petA-psbJ_p2	CGTCCAAGGCCTTTTGTTCT	TCTGGATTGGGTTCGTCTCT	50.0	50.0	58.7	58.1	1046-1073 bp	1.0	76.7
petA-psbJ_p3	GGCCTTTTGTTCTTCTTCGC	TTCTGGATTGGGTTCGTCTC	50.0	50.0	57.9	57.2	1040-1067 bp	1.0	76.1
petA-psbJ_p4	GCCTTTTGTTCTTCTTCGCA	TCTGGATTGGGTTCGTCTCT	45.0	50.0	57.2	58.1	1038-1065 bp	1.0	76.0
petA-psbJ_p5	GGCCTTTTGTTCTTCTTCGC	TCTGGATTGGGTTCGTCTCTA	50.0	47.6	57.9	57.9	1039-1066 bp	1.0	73.5