Hotspot

trnD-GUC-psbM

LSC · 15435-16360 · 926 bp

Back to Rytidosperma

Pi 0.00221
MarkerSeek score 62.00
Primer available yes
Label trnD-GUC-psbM

Feature Metadata

label_name
trnD-GUC-psbM
feature_type
igs
region
LSC
length_bp
926
variable_sites
2
pi
0.00221
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-psbM_p1 TTCAATTGGTCAGAGCACCG ATTAACCCTTTTGGCTGGCT 50.0 45.0 58.5 57.7 1077-1096 bp 1.0 75.1
trnD-GUC-psbM_p2 TTCAATTGGTCAGAGCACCG CCTTTTGGCTGGCTGTTTTT 50.0 45.0 58.5 57.7 1071-1090 bp 1.0 75.1
trnD-GUC-psbM_p3 CCTGCAGGGATTGTAGTTCA ATTAACCCTTTTGGCTGGCT 50.0 45.0 57.2 57.7 1093-1112 bp 1.0 72.6
trnD-GUC-psbM_p4 CCTGCAGGGATTGTAGTTCA CCTTTTGGCTGGCTGTTTTT 50.0 45.0 57.2 57.7 1087-1106 bp 1.0 72.6
trnD-GUC-psbM_p5 TTCAATTGGTCAGAGCACCG TTAACCCTTTTGGCTGGCTG 50.0 50.0 58.5 58.7 1076-1095 bp 1.0 72.4