Hotspot

trnG-UCC-trnM-CAU

LSC · 13615-14226 · 612 bp

Back to Rytidosperma

Pi 0.00654

MarkerSeek score 62.50

Primer available yes

Label trnG-UCC-trnM-CAU

Feature Metadata

label_name: trnG-UCC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 612
variable_sites: 4
pi: 0.00654
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnM-CAU_p1	ACGAATCACACTTTTACCACT	CCGGTGCATTACTCTACCAC	38.1	55.0	55.5	58.1	741-762 bp	1.0	51.6
trnG-UCC-trnM-CAU_p2	AGAACGAATCACACTTTTACCAC	CCGGTGCATTACTCTACCAC	39.1	55.0	57.3	58.1	744-765 bp	1.0	50.6
trnG-UCC-trnM-CAU_p3	GAACGAATCACACTTTTACCACT	CCGGTGCATTACTCTACCAC	39.1	55.0	57.3	58.1	743-764 bp	1.0	50.6
trnG-UCC-trnM-CAU_p4	AACGAATCACACTTTTACCACT	CCGGTGCATTACTCTACCAC	36.4	55.0	56.2	58.1	742-763 bp	1.0	49.7
trnG-UCC-trnM-CAU_p5	ACGAATCACACTTTTACCACT	ACCGGTGCATTACTCTACCA	38.1	50.0	55.5	58.4	742-763 bp	1.0	48.6