Hotspot

psbZ-trnG-GCC

LSC · 37871-38418 · 548 bp

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Pi 0.0172
MarkerSeek score 62.50
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
548
variable_sites
9
pi
0.0172
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 AGTCTTTCTGGTGGGTATCCT CGTCTTCTCCTTGGCAAAGA 47.6 50.0 57.8 57.8 629-635 bp 1.0 73.4
psbZ-trnG-GCC_p2 AGTCTTTCTGGTGGGTATCC CGTCTTCTCCTTGGCAAAGA 50.0 50.0 56.2 57.8 629-635 bp 1.0 68.9
psbZ-trnG-GCC_p3 GTCTTTCTGGTGGGTATCCT CGTCTTCTCCTTGGCAAAGA 50.0 50.0 56.2 57.8 628-634 bp 1.0 68.9
psbZ-trnG-GCC_p4 GTCTTTCTGGTGGGTATCCTT CGTCTTCTCCTTGGCAAAGA 47.6 50.0 57.0 57.8 628-634 bp 1.0 67.1
psbZ-trnG-GCC_p5 TGGATTAGTCTTTCTGGTGGG CGTCTTCTCCTTGGCAAAGA 47.6 50.0 57.0 57.8 635-641 bp 1.0 67.0