Hotspot

atpI-rps2

LSC · 15854-16086 · 233 bp

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Pi 0.0258
MarkerSeek score 67.30
Primer available yes
Label atpI-rps2

Feature Metadata

label_name
atpI-rps2
feature_type
igs
region
LSC
length_bp
233
variable_sites
6
pi
0.0258
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI-rps2_p1 ATGGACTTGGAACCCACCTA GACGCTATAGCCTCAATCCG 50.0 55.0 57.9 58.0 428 bp 1.0 80.6
atpI-rps2_p2 TGGACTTGGAACCCACCTAT GACGCTATAGCCTCAATCCG 50.0 55.0 57.9 58.0 427 bp 1.0 80.6
atpI-rps2_p3 GGAACCCACCTATTTGCCAA GACGCTATAGCCTCAATCCG 50.0 55.0 58.1 58.0 420 bp 1.0 80.5
atpI-rps2_p4 TTGGAACCCACCTATTTGCC GACGCTATAGCCTCAATCCG 50.0 55.0 58.1 58.0 422 bp 1.0 80.5
atpI-rps2_p5 ATGTTGGCCTACTTCCACAC GACGCTATAGCCTCAATCCG 50.0 55.0 57.8 58.0 395 bp 1.0 79.5