Hotspot

psbK-psbI

LSC · 7327-7740 · 414 bp

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Pi 0.0152
MarkerSeek score 63.60
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
414
variable_sites
6
pi
0.0152
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 GTTTGGCAAGCTGCTGTAAG CGCCCTGGATCATTAGACAG 50.0 55.0 57.9 57.8 515-533 bp 1.0 81.9
psbK-psbI_p2 TGGCAAGCTGCTGTAAGTTT CGCCCTGGATCATTAGACAG 45.0 55.0 58.3 57.8 512-530 bp 1.0 79.9
psbK-psbI_p3 TTGGCAAGCTGCTGTAAGTT CGCCCTGGATCATTAGACAG 45.0 55.0 58.3 57.8 513-531 bp 1.0 79.9
psbK-psbI_p4 TTTGGCAAGCTGCTGTAAGT CGCCCTGGATCATTAGACAG 45.0 55.0 58.3 57.8 514-532 bp 1.0 79.9
psbK-psbI_p5 GTTTGGCAAGCTGCTGTAAG ACGCCCTGGATCATTAGACA 50.0 50.0 57.9 58.5 516-534 bp 1.0 79.2