Hotspot

rps16-trnQ-UUG

LSC · 5992-6701 · 710 bp

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Pi 0.0183
MarkerSeek score 67.20
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
710
variable_sites
13
pi
0.0183
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CAGCGGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 801-804 bp 1.0 80.6
rps16-trnQ-UUG_p2 GCGGATCATGTCCTTCAAGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 799-802 bp 1.0 80.6
rps16-trnQ-UUG_p3 CAGCGGATCATGTCCTTCAA GAAATTGAAATGGGGCGTCG 50.0 50.0 58.0 58.1 859-862 bp 1.0 80.3
rps16-trnQ-UUG_p4 GCGGATCATGTCCTTCAAGT GAAATTGAAATGGGGCGTCG 50.0 50.0 58.0 58.1 857-860 bp 1.0 80.3
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 783-786 bp 1.0 79.7