Hotspot

rpl2-rpl23

IRb · 85074-85097 · 24 bp

Back to Ruscus

Pi 0.0833
MarkerSeek score 85.40
Primer available yes
Label rpl2-rpl23

Feature Metadata

label_name
rpl2-rpl23
feature_type
igs
region
IRb
length_bp
24
variable_sites
2
pi
0.0833
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
85.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl2-rpl23_p1 CACTTGACTGTCTACGGCTC CGCTTCAACCGGGTTATTCT 55.0 50.0 58.0 58.3 134 bp 1.0 78.9
rpl2-rpl23_p2 CCACAACGATGCTGTCCATA CGCTTCAACCGGGTTATTCT 50.0 50.0 58.0 58.3 181 bp 1.0 78.6
rpl2-rpl23_p3 GACTGTCTACGGCTCCATTG CGCTTCAACCGGGTTATTCT 55.0 50.0 58.1 58.3 129 bp 1.0 78.5
rpl2-rpl23_p4 CACTTGACTGTCTACGGCTC ATCATTACGCTTCAACCGGG 55.0 50.0 58.0 58.3 141 bp 1.0 78.3
rpl2-rpl23_p5 CCACAACGATGCTGTCCATA ATCATTACGCTTCAACCGGG 50.0 50.0 58.0 58.3 188 bp 1.0 78.0