Hotspot

trnT-GGU-psbD

LSC · 30831-31593 · 763 bp

Back to Ruscus

Pi 0.00131
MarkerSeek score 59.50
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
763
variable_sites
1
pi
0.00131
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG GGACCAGCCTACAAAAACGA 55.0 50.0 58.1 58.1 923-924 bp 1.0 78.7
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA GGACCAGCCTACAAAAACGA 50.0 50.0 58.1 58.1 926-927 bp 1.0 78.5
trnT-GGU-psbD_p3 GGCGTAAGTCATCGGTTCAA GGACCAGCCTACAAAAACGA 50.0 50.0 58.3 58.1 900-901 bp 1.0 76.9
trnT-GGU-psbD_p4 GGCCCTTTTAACTCAGTGGT GGACCAGCCTACAAAAACGA 50.0 50.0 57.7 58.1 938-939 bp 1.0 76.9
trnT-GGU-psbD_p5 TGGTAGAGTAACGCCATGGT GGACCAGCCTACAAAAACGA 50.0 50.0 58.4 58.1 922-923 bp 1.0 75.7