Hotspot

trnC-GCA-petN

LSC · 26918-27837 · 920 bp

Back to Ruscus

Pi 0.00109
MarkerSeek score 59.40
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
920
variable_sites
1
pi
0.00109
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 ATTTTGGACCGAATTTGGCG TTAAAGCAGCCCAAGCAAGA 45.0 45.0 57.6 58.0 1041 bp 1.0 77.1
trnC-GCA-petN_p2 ATTTTGGACCGAATTTGGCG AGCAATTAGTAGTGCCCAGC 45.0 50.0 57.6 58.0 1118 bp 1.0 76.8
trnC-GCA-petN_p3 ATTTTGGACCGAATTTGGCG AGTCCACTCCTTCCCCATAC 45.0 55.0 57.6 58.1 1093 bp 1.0 76.2
trnC-GCA-petN_p4 ATTTTGGACCGAATTTGGCG GTCCACTCCTTCCCCATACT 45.0 55.0 57.6 58.1 1092 bp 1.0 76.2
trnC-GCA-petN_p5 GGGGGACTGCAAATCCTTTA TTAAAGCAGCCCAAGCAAGA 50.0 45.0 57.5 58.0 1001 bp 1.0 75.8