Hotspot

atpA-atpF

LSC · 10702-10775 · 74 bp

Back to Ruscus

Pi 0.0135
MarkerSeek score 60.30
Primer available yes
Label atpA-atpF

Feature Metadata

label_name
atpA-atpF
feature_type
igs
region
LSC
length_bp
74
variable_sites
1
pi
0.0135
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpA-atpF_p1 CCGTCGCCTACTTGAAGTAC CGGGGCCATGGAAGAAATAA 55.0 50.0 58.1 57.9 215 bp 1.0 79.1
atpA-atpF_p2 GTCGCCTACTTGAAGTACGG CGGGGCCATGGAAGAAATAA 55.0 50.0 58.1 57.9 213 bp 1.0 79.1
atpA-atpF_p3 CCGTCGCCTACTTGAAGTAC AGCCTTACAGGGAGCTCTAG 55.0 55.0 58.1 57.9 302 bp 1.0 78.9
atpA-atpF_p4 GTCGCCTACTTGAAGTACGG AGCCTTACAGGGAGCTCTAG 55.0 55.0 58.1 57.9 300 bp 1.0 78.9
atpA-atpF_p5 GTCGCCTACTTGAAGTACGG GCGAGTTACATCTCCGTACC 55.0 55.0 58.1 57.9 256 bp 1.0 78.6