Hotspot

trnK-UUU-trnQ-UUG

LSC · 4025-5711 · 1687 bp

Back to Ruscus

Pi 0.000593
MarkerSeek score 58.70
Primer available yes
Label trnK-UUU-trnQ-UUG

Feature Metadata

label_name
trnK-UUU-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1687
variable_sites
1
pi
0.000593
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-trnQ-UUG_p1 AAAGCCGAGTACTCTACCGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1745 bp 1.0 78.1
trnK-UUU-trnQ-UUG_p2 AAGCCGAGTACTCTACCGTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1744 bp 1.0 78.1
trnK-UUU-trnQ-UUG_p3 TACCGTTGAGTTAGCAACCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.5 58.1 1731 bp 1.0 75.8
trnK-UUU-trnQ-UUG_p4 AGTCGCACTTAAAAGCCGAG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.6 58.1 1756 bp 1.0 74.9
trnK-UUU-trnQ-UUG_p5 TCGCACTTAAAAGCCGAGTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.3 58.1 1754 bp 1.0 73.6