Hotspot

ccsA

SSC · 118838-119803 · 966 bp

Pi 0.00483

MarkerSeek score 46.80

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 966
variable_sites: 7
pi: 0.00483
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	ATTTCAAACAAGCCGCCATG	TGGACCACGCGAACTTTTTA	45.0	45.0	57.9	58.0	1520-1534 bp	1.0	80.7
ccsA_p2	TAGACACGCTGCTCTTAGGA	TGGACCACGCGAACTTTTTA	50.0	45.0	57.9	58.0	1490-1504 bp	1.0	80.4
ccsA_p3	AAATTGGTAGACACGCTGCT	TGGACCACGCGAACTTTTTA	45.0	45.0	57.8	58.0	1497-1511 bp	1.0	79.9
ccsA_p4	GGTAGACACGCTGCTCTTAG	TGGACCACGCGAACTTTTTA	55.0	45.0	57.8	58.0	1492-1506 bp	1.0	79.8
ccsA_p5	GTAGACACGCTGCTCTTAGG	TGGACCACGCGAACTTTTTA	55.0	45.0	57.8	58.0	1491-1505 bp	1.0	79.8