Hotspot

petG-trnW-CCA

LSC · 70604-70710 · 107 bp

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Pi 0.0249
MarkerSeek score 62.50
Primer available yes
Label petG-trnW-CCA

Feature Metadata

label_name
petG-trnW-CCA
feature_type
igs
region
LSC
length_bp
107
variable_sites
4
pi
0.0249
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petG-trnW-CCA_p1 ACAGACGTGGTGATCAGTTG CAAAACCCGATGTCGTAGGT 50.0 50.0 57.8 57.9 175 bp 1.0 79.9
petG-trnW-CCA_p2 ACAGACGTGGTGATCAGTTG TTCAAATCCTACAGGGCGTG 50.0 50.0 57.8 57.9 156 bp 1.0 79.9
petG-trnW-CCA_p3 ACAGACGTGGTGATCAGTTG GTTCAAATCCTACAGGGCGT 50.0 50.0 57.8 57.9 157 bp 1.0 79.9
petG-trnW-CCA_p4 GACGTGGTGATCAGTTGGAT CAAAACCCGATGTCGTAGGT 50.0 50.0 57.6 57.9 172 bp 1.0 78.1
petG-trnW-CCA_p5 GACGTGGTGATCAGTTGGAT TTCAAATCCTACAGGGCGTG 50.0 50.0 57.6 57.9 153 bp 1.0 78.1