Hotspot

petA-psbJ

LSC · 67243-68252 · 1010 bp

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Pi 0.0152
MarkerSeek score 64.50
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1010
variable_sites
23
pi
0.0152
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 CCCATTACGTGTCCAAGGTC TTCCGGATTAGGCTCATCCC 55.0 55.0 58.3 58.9 1153-1159 bp 1.0 71.9
petA-psbJ_p2 GACCCATTACGTGTCCAAGG TTCCGGATTAGGCTCATCCC 55.0 55.0 58.3 58.9 1155-1161 bp 1.0 71.9
petA-psbJ_p3 CCCATTACGTGTCCAAGGTC CGGATTAGGCTCATCCCTGT 55.0 55.0 58.3 58.9 1150-1156 bp 1.0 71.9
petA-psbJ_p4 GACCCATTACGTGTCCAAGG CGGATTAGGCTCATCCCTGT 55.0 55.0 58.3 58.9 1152-1158 bp 1.0 71.9
petA-psbJ_p5 ACGTGTCCAAGGTCTTTTGT TTCCGGATTAGGCTCATCCC 45.0 55.0 57.6 58.9 1147-1153 bp 1.0 70.7