Hotspot

psaA-ycf3

LSC · 45148-45845 · 698 bp

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Pi 0.0105
MarkerSeek score 66.40
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
698
variable_sites
11
pi
0.0105
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 GTTTTTGAGAAATGCCCCGG CAGAGGCTTGGTTCGATCAA 50.0 50.0 57.9 57.9 910-915 bp 1.0 80.1
psaA-ycf3_p2 CCCATTCCTCGAAAGACGTT CAGAGGCTTGGTTCGATCAA 50.0 50.0 57.9 57.9 885-890 bp 1.0 80.0
psaA-ycf3_p3 GTTTTTGAGAAATGCCCCGG ATAGCGCTTACTCCGGGTAA 50.0 50.0 57.9 58.3 866-871 bp 1.0 78.5
psaA-ycf3_p4 GTTTTTGAGAAATGCCCCGG TAGCGCTTACTCCGGGTAAT 50.0 50.0 57.9 58.3 865-870 bp 1.0 78.5
psaA-ycf3_p5 TTTTTGAGAAATGCCCCGGT CAGAGGCTTGGTTCGATCAA 45.0 50.0 58.3 57.9 909-914 bp 1.0 78.4