Hotspot

psbI-trnS-GCU

LSC · 9648-9784 · 137 bp

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Pi 0.0276
MarkerSeek score 65.80
Primer available yes
Label psbI-trnS-GCU

Feature Metadata

label_name
psbI-trnS-GCU
feature_type
igs
region
LSC
length_bp
137
variable_sites
5
pi
0.0276
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS-GCU_p1 ATGATCCCGGACGTAATCCT TGGACTAAAGCGTCGGATTG 50.0 50.0 58.0 58.0 243-245 bp 1.0 83.5
psbI-trnS-GCU_p2 ATGATCCCGGACGTAATCCT GTGGACTAAAGCGTCGGATT 50.0 50.0 58.0 58.0 244-246 bp 1.0 83.5
psbI-trnS-GCU_p3 ATGATCCCGGACGTAATCCT GGGTTCGAATCCCTCTCTCT 50.0 55.0 58.0 58.2 193-195 bp 1.0 82.7
psbI-trnS-GCU_p4 ATGATCCCGGACGTAATCCT ATTGGGAGAGATGGCTGAGT 50.0 50.0 58.0 58.1 262-264 bp 1.0 82.4
psbI-trnS-GCU_p5 ATGATCCCGGACGTAATCCT TTCGAATCCCTCTCTCTCCG 50.0 55.0 58.0 58.3 190-192 bp 1.0 82.0