Hotspot

psbK-psbI

LSC · 9060-9536 · 477 bp

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Pi 0.0151
MarkerSeek score 55.60
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
477
variable_sites
10
pi
0.0151
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.93
markerseek_score
55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 GTTTGGCAAGCTGCTGTAAG TGGACTAAAGCGTCGGATTG 50.0 50.0 57.9 58.0 790-828 bp 1.0 85.1
psbK-psbI_p2 GTTTGGCAAGCTGCTGTAAG GTGGACTAAAGCGTCGGATT 50.0 50.0 57.9 58.0 791-829 bp 1.0 85.1
psbK-psbI_p3 GTTTGGCAAGCTGCTGTAAG CCGGGGGAATCAAAAAGGAA 50.0 50.0 57.9 58.1 972-1016 bp 1.0 84.5
psbK-psbI_p4 GTTTGGCAAGCTGCTGTAAG ATTGGGAGAGATGGCTGAGT 50.0 50.0 57.9 58.1 809-847 bp 1.0 84.2
psbK-psbI_p5 TTTTCTTCGCCAAATTGCCC TGGACTAAAGCGTCGGATTG 45.0 50.0 57.8 58.0 885-923 bp 1.0 84.1