Hotspot

rps16-trnQ-UUG

LSC · 6522-8111 · 1590 bp

Back to Ruppia

Pi 0.016
MarkerSeek score 64.10
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1590
variable_sites
38
pi
0.016
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 GCCAAGAGCATTTTCGTTCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1729-1786 bp 1.0 81.3
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1663-1720 bp 1.0 80.4
rps16-trnQ-UUG_p3 TGATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1674-1731 bp 1.0 79.9
rps16-trnQ-UUG_p4 GCCAAGAGCATTTTCGTTCC TAAATCGAAATGGGGCGTGG 50.0 50.0 58.0 58.6 1787-1844 bp 1.0 76.9
rps16-trnQ-UUG_p5 GCCAAGAGCATTTTCGTTCC TTCGGAGGTTCGAATCCTTC 50.0 50.0 58.0 57.3 1733-1790 bp 1.0 76.6