Hotspot

trnK-UUU-rps16

LSC · 4167-5168 · 1002 bp

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Pi 0.0108
MarkerSeek score 67.40
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1002
variable_sites
16
pi
0.0108
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGTCGCACTTAAAAGCCGAG CAACCCACAGAGACGGTTTA 50.0 50.0 58.6 57.5 1125-1134 bp 1.0 73.6
trnK-UUU-rps16_p2 TAGTCGCACTTAAAAGCCGA CAACCCACAGAGACGGTTTA 45.0 50.0 57.3 57.5 1126-1135 bp 1.0 72.3
trnK-UUU-rps16_p3 TCGCACTTAAAAGCCGAGTA CAACCCACAGAGACGGTTTA 45.0 50.0 57.3 57.5 1123-1132 bp 1.0 72.3
trnK-UUU-rps16_p4 CGCACTTAAAAGCCGAGTAC CAACCCACAGAGACGGTTTA 50.0 50.0 57.2 57.5 1122-1131 bp 1.0 71.9
trnK-UUU-rps16_p5 AGTCGCACTTAAAAGCCGAG ACCCACAGAGACGGTTTATG 50.0 50.0 58.6 57.2 1123-1132 bp 1.0 71.7