Hotspot

ndhF-rpl32

SSC · 117515-118631 · 1117 bp

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Pi 0.0293
MarkerSeek score 71.30
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1117
variable_sites
106
pi
0.0293
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CCGCTCCGATTAACAAAGGA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.0 58.0 1752-1870 bp 1.0 91.2
ndhF-rpl32_p2 CCGCTCCGATTAACAAAGGA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.0 58.0 1753-1871 bp 1.0 91.2
ndhF-rpl32_p3 CCCACACGGTTAGTCACAAA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.0 58.0 2233-2351 bp 1.0 90.0
ndhF-rpl32_p4 CCCACACGGTTAGTCACAAA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.0 58.0 2234-2352 bp 1.0 89.9
ndhF-rpl32_p5 ACGGTTAGTCACAAACGCTT AGCGTGTCTACCGATTTCAC 45.0 50.0 58.1 58.0 2228-2346 bp 1.0 89.8