Hotspot

trnT-UGU-trnL-UAA

LSC · 47040-47674 · 635 bp

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Pi 0.0302
MarkerSeek score 74.80
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
635
variable_sites
61
pi
0.0302
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 TGAAGCGAAATCCCCTGAAG TTTGGAAAGGAGGAGTTGGC 50.0 50.0 57.9 58.0 1409-1591 bp 1.0 91.1
trnT-UGU-trnL-UAA_p2 GAAGCGAAATCCCCTGAAGT TTTGGAAAGGAGGAGTTGGC 50.0 50.0 57.9 58.0 1408-1590 bp 1.0 91.0
trnT-UGU-trnL-UAA_p3 TGAAGCGAAATCCCCTGAAG GAGGAGTTGGCTCAGGATTG 50.0 55.0 57.9 58.0 1400-1582 bp 1.0 90.8
trnT-UGU-trnL-UAA_p4 GAAGCGAAATCCCCTGAAGT GAGGAGTTGGCTCAGGATTG 50.0 55.0 57.9 58.0 1399-1581 bp 1.0 90.8
trnT-UGU-trnL-UAA_p5 GCGATGCTCTAACCTCTGAG TTTGGAAAGGAGGAGTTGGC 55.0 50.0 57.9 58.0 809-970 bp 1.0 87.5