Hotspot

trnT-GGU-psbD

LSC · 31461-32676 · 1216 bp

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Pi 0.0384
MarkerSeek score 77.70
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1216
variable_sites
145
pi
0.0384
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG TTTACCAAGGGCTATAGTCA 55.0 40.0 58.1 53.0 1255-1461 bp 1.0 52.0
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA TTTACCAAGGGCTATAGTCA 50.0 40.0 58.1 53.0 1258-1464 bp 1.0 52.0
trnT-GGU-psbD_p3 GGCGTAAGTCATCGGTTCAA TTTACCAAGGGCTATAGTCA 50.0 40.0 58.3 53.0 1232-1438 bp 1.0 52.0
trnT-GGU-psbD_p4 GTGGTAGAGTAACGCCATGG TTACCAAGGGCTATAGTCAT 55.0 40.0 58.1 52.8 1254-1460 bp 1.0 52.0
trnT-GGU-psbD_p5 TCAGTGGTAGAGTAACGCCA TTACCAAGGGCTATAGTCAT 50.0 40.0 58.1 52.8 1257-1463 bp 1.0 52.0