Hotspot

trnC-GCA-petN

LSC · 27446-28031 · 586 bp

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Pi 0.0314
MarkerSeek score 79.40
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
586
variable_sites
55
pi
0.0314
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 AGTTCAAATCTGGGTGTCGC AGCCCAAGCGAGACTTACTA 50.0 50.0 58.5 58.2 590-639 bp 1.0 86.0
trnC-GCA-petN_p2 TTCAAATCTGGGTGTCGCC AGCCCAAGCGAGACTTACTA 52.6 50.0 58.1 58.2 588-637 bp 1.0 81.4
trnC-GCA-petN_p3 AGTTCAAATCTGGGTGTCGC GCCCAAGCGAGACTTACTAT 50.0 50.0 58.5 57.1 589-638 bp 1.0 80.1
trnC-GCA-petN_p4 AGTTCAAATCTGGGTGTCGC ATTAAAGCAGCCCAAGCGAG 50.0 50.0 58.5 58.9 598-647 bp 1.0 79.9
trnC-GCA-petN_p5 CAGTTCAAATCTGGGTGTCG AGCCCAAGCGAGACTTACTA 50.0 50.0 56.7 58.2 591-640 bp 1.0 79.7