Hotspot

trnS-GCU-trnG-UCC

LSC · 7870-8655 · 786 bp

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Pi 0.0421
MarkerSeek score 79.20
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
786
variable_sites
94
pi
0.0421
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.98
markerseek_score
79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 ACGGAAAGAGAGGGATTCGA ACGAATCACACTTTTACCACT 50.0 38.1 58.2 55.5 909-1368 bp 1.0 61.6
trnS-GCU-trnG-UCC_p2 CTCGTACAGCGGATTAGCAA ACGAATCACACTTTTACCACT 50.0 38.1 57.8 55.5 872-1331 bp 1.0 61.5
trnS-GCU-trnG-UCC_p3 GACTCGTACAGCGGATTAGC ACGAATCACACTTTTACCACT 55.0 38.1 58.2 55.5 874-1333 bp 1.0 61.2
trnS-GCU-trnG-UCC_p4 ACGGAAAGAGAGGGATTCGA AGAACGAATCACACTTTTACCAC 50.0 39.1 58.2 57.3 912-1371 bp 1.0 60.6
trnS-GCU-trnG-UCC_p5 ACGGAAAGAGAGGGATTCGA GAACGAATCACACTTTTACCACT 50.0 39.1 58.2 57.3 911-1370 bp 1.0 60.6