Hotspot

trnH-GUG-psbA

LSC · 78-315 · 238 bp

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Pi 0.086
MarkerSeek score 77.90
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
238
variable_sites
55
pi
0.086
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.97
markerseek_score
77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG ATTTCAGGCTGAGCACAACA 55.0 45.0 58.1 58.0 760-792 bp 1.0 88.0
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG TTTCAGGCTGAGCACAACAT 55.0 45.0 58.1 58.0 759-791 bp 1.0 88.0
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA ATTTCAGGCTGAGCACAACA 50.0 45.0 57.9 58.0 774-806 bp 1.0 87.8
trnH-GUG-psbA_p4 CAATCCACTGCCTTGATCCA TTTCAGGCTGAGCACAACAT 50.0 45.0 57.9 58.0 773-805 bp 1.0 87.8
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG CTGTTGCGTATTCAGCTCCT 55.0 50.0 58.1 58.0 884-916 bp 1.0 86.8