Hotspot

ycf1

SSC · 108059-113509 · 5451 bp

Back to Ruellia

Pi 0.00516

MarkerSeek score 55.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5451
variable_sites: 28
pi: 0.00516
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGCTGTGAGAAACGAGATGT	TTGATTATGCCTCGCCGAAA	50.0	45.0	57.9	58.0	942-948 bp	1.0	80.1
ycf1_p2	GGCTGTGAGAAACGAGATGT	TTTGATTATGCCTCGCCGAA	50.0	45.0	57.9	58.0	943-949 bp	1.0	80.1
ycf1_p3	GGCTGTGAGAAACGAGATGT	GACCATTCAGGAGGTTGCAA	50.0	50.0	57.9	58.1	1344-1350 bp	1.0	79.4
ycf1_p4	GGCTGTGAGAAACGAGATGT	CGAAAGTCGGATTGTTGGGA	50.0	50.0	57.9	58.2	927-933 bp	1.0	78.8
ycf1_p5	TTCGATTCGAAGCGATGGAA	TTGATTATGCCTCGCCGAAA	45.0	45.0	57.7	58.0	1001-1007 bp	1.0	78.4