Hotspot

trnN-GUU-trnR-ACG

IRa · 127643-128205 · 563 bp

Back to Ruellia

Pi 0.0054

MarkerSeek score 62.30

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 563
variable_sites: 3
pi: 0.0054
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	697-704 bp	1.0	79.4
trnN-GUU-trnR-ACG_p2	CCGTTGTTCCTCAGTAGCTC	GAACAGATCCAGTGGAGACG	55.0	55.0	58.0	57.7	734-741 bp	1.0	78.8
trnN-GUU-trnR-ACG_p3	TGGGTTGAGAACGGGAATTG	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.1	57.8	738-745 bp	1.0	78.6
trnN-GUU-trnR-ACG_p4	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	648-655 bp	1.0	78.2
trnN-GUU-trnR-ACG_p5	TGGGTTGAGAACGGGAATTG	GAACAGATCCAGTGGAGACG	50.0	55.0	58.1	57.7	775-782 bp	1.0	78.0