Hotspot

rpl32-ndhF

SSC · 123761-124286 · 526 bp

Back to Ruellia

Pi 0.01

MarkerSeek score 61.80

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 526
variable_sites: 5
pi: 0.01
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	TTAACGCTGCCCAATATCCC	ACTGGAAGCAGAACGAAAGG	50.0	50.0	58.0	58.1	641-667 bp	1.0	82.7
rpl32-ndhF_p2	TTAACGCTGCCCAATATCCC	CTGGAAGCAGAACGAAAGGT	50.0	50.0	58.0	58.1	640-666 bp	1.0	82.7
rpl32-ndhF_p3	TTAACGCTGCCCAATATCCC	GGAACTGGAAGCAGAACGAA	50.0	50.0	58.0	58.1	644-670 bp	1.0	82.6
rpl32-ndhF_p4	GCTGCCCAATATCCCTTTCT	ACTGGAAGCAGAACGAAAGG	50.0	50.0	57.6	58.1	636-662 bp	1.0	79.9
rpl32-ndhF_p5	GCTGCCCAATATCCCTTTCT	CTGGAAGCAGAACGAAAGGT	50.0	50.0	57.6	58.1	635-661 bp	1.0	79.9