Hotspot

ndhD-ccsA

SSC · 121737-121990 · 254 bp

Back to Ruellia

Pi 0.0315

MarkerSeek score 70.60

Primer available yes

Label ndhD-ccsA

Feature Metadata

label_name: ndhD-ccsA
feature_type: igs
region: SSC
length_bp: 254
variable_sites: 8
pi: 0.0315
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-ccsA_p1	ACCCCGATTTCGTTCTTTCA	GCAATTGTAGCGTCTATTGGC	45.0	47.6	57.4	58.0	436 bp	1.0	68.9
ndhD-ccsA_p2	TGGTATGTACCCCGATTTCG	GCAATTGTAGCGTCTATTGGC	50.0	47.6	57.1	58.0	444 bp	1.0	66.2
ndhD-ccsA_p3	GGTATGTACCCCGATTTCGT	GCAATTGTAGCGTCTATTGGC	50.0	47.6	57.1	58.0	443 bp	1.0	66.2
ndhD-ccsA_p4	ACCCCGATTTCGTTCTTTCAT	GCAATTGTAGCGTCTATTGGC	42.9	47.6	57.9	58.0	436 bp	1.0	64.8
ndhD-ccsA_p5	TGTACCCCGATTTCGTTCTT	GCAATTGTAGCGTCTATTGGC	45.0	47.6	56.9	58.0	439 bp	1.0	64.3