Hotspot

rps19-rpl2

LSC · 83437-83504 · 68 bp

Back to Ruellia

Pi 0.0317

MarkerSeek score 74.60

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: LSC
length_bp: 68
variable_sites: 2
pi: 0.0317
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	TGCACGGGACCAAGTTACTA	GATTGTTCGTCGCCGTAGTA	50.0	50.0	58.7	57.8	199-204 bp	1.0	76.1
rps19-rpl2_p2	GCACGGGACCAAGTTACTAT	GATTGTTCGTCGCCGTAGTA	50.0	50.0	57.3	57.8	198-203 bp	1.0	75.9
rps19-rpl2_p3	TGCACGGGACCAAGTTACTA	CACAACCCCTTGGGGTTATC	50.0	55.0	58.7	58.2	268-273 bp	1.0	75.6
rps19-rpl2_p4	GCACGGGACCAAGTTACTAT	CACAACCCCTTGGGGTTATC	50.0	55.0	57.3	58.2	267-272 bp	1.0	75.4
rps19-rpl2_p5	TGCACGGGACCAAGTTACTA	TTGGGGTTATCCTGCACTTG	50.0	50.0	58.7	57.8	259-264 bp	1.0	75.1